The comprehensive quality evaluation of scutellariae radix based on HPLC fingerprint and antibacterial activity

Scutellaria Radix, a traditional Chinese medicine, studies on its main active ingredient are limited. In this study, the purpose was to investigate quality difference of Scutellariae Radix from different origins based chemical components and biological activities. The chromatographic fingerprints 33 were established using HPLC, antibacterial activities studied with microdilution method. Moreover, orthogonal partial least-square regression, pearson correlational analysis grey relational methods performed explore relationship between compositions bioactivities. addition, origin identification model comprehensively evaluate Radix. results showed that had in-vitro effect Staphylococcus aureus, best in Gansu, Shandong Province. Multivariate statistical common three positively correlated activity, which respectively wogonin, baicalein oroxylin. conclusion, varies greatly origins, better Gansu This work successfully provides general combined fingerprint bioactivity assay study spectrum–effect relationships, could be used discover primary ingredients medicines.